Do Wait for The Train Correctly

The platform has handy marks to show where each train door will be. Locals stand in two neat lines, one on each side of the door (or four lines during busy times).
When the train comes, the people in the train exit first. Once everyone has gotten off, the lines will file inside.

Do not Eat on The Train

The only time where eating and drinking on trains is acceptable is on regional long-distance trains, like the Shinkansen, where every seat has a tray and cup holder. If you’re hungry, you can eat a snack at the platform or visit a small noodle shop often found inside the station.
If you must eat, choose a small snack or some bakery bread (nothing smelly) and try to minimize chewing noises.

Do not Talk on The Train in Loud Voice

The best volume for train conversations is just low enough that other people can’t discern what you’re saying. However, talking on the phone is never appropriate, except for emergency situations.If you get a call while riding, mute the phone, get off at the next stop and call the person back.

Do Know the Designated areas on The Train

Most train cars have “Priority Seating.” These are for pregnant women, disabled or elderly passengers, or people with small children.
You can sit in these seats if they’re open, but if you see someone who needs to sit down, make sure to offer them your seat.
Some of the most popular train lines have women-only cars during rush hour to prevent women from getting groped on the train. This car is also open to children, the disabled, and male caretakers of the disabled. If you’re not female, make sure that you don’t get on one of these cars during the designated women-only hours, or you may have a horde of angry females staring at you.

Do Not Put on Makeup on The Train

Japan consider it inappropriate to fix your hair or makeup on the train. Some consider it to be sloppy. Others find flying powders or strong smells problematic. Some see fixing your makeup as an intimate act similar to putting clothes on.
Putting on lip balm or fixing your lipstick won’t turn any heads. But you may get stares if you do your entire hair or makeup routine on the train.

Do you agree or disagree with these manners and rules?

Source: FUN! JAPAN!